Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion
| Abstract | Stimulated emission depletion (STED) is the key optical technologyenabling super-resolution microscopy below the diffraction limit. Here,we demonstrate that modulation of STED in the time domain, combined withproperly designed lock-in detection, can radically enhance the contrastof fluorescent images of strongly autofluorescent biotissues. In ourexperiments, the temporally modulated STED technique, implemented withlow-intensity continuous-wave laser sources, is shown to provide anefficient all-optical suppression of a broadband fluorescent background,allowing the contrast of fluorescent images of mammal brain tissuestagged with nitrogen-vacancy diamond to be increased by five orders ofmagnitude. |
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| Authors | A.M. Zheltikov, I.V. Fedotov, L.V. Doronina-Amitonova |
| Year of publication | 2015 |
| Date published | 02/2015 |
| Journal | Opt. Lett. |
| Volume | 40 |
| Pages | 725-728 |
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